Non-testing methods including QSARs and read across are regarded as potential approaches to support the risk assessment of chemicals for generating information on the intrinsic properties of chemicals. They are all based on the notion that similar compounds (usually structurally similar chemicals) have similar activities, which can be predicted based on chemical structure or analogue chemicals. Traditional experimental measurements of toxicity are usually costly and violating the 3R principles of animal test. Thus, non-animal methods are encouraged to fill in data gaps by Organization for Economic Cooperation and Development (OECD) and United States Environmental Protection Agency (US EPA), and regulatory frameworks are providing an increasing responsibility to use such methods. Read-across is probably the simplest tool to reduce animal testing under the regulatory. In read-across, one or more properties of a chemical of interest are inferred by comparison to a similar chemical or chemicals, for which the properties of interest…
